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This article has been cited by other articles in PMC. Abstract Objective To examine the diagnostic performance of real-time reverse transcription RT -polymerase chain reaction PCR assays for Zika virus detection.
Findings Oligonucleotides of the published real-time RT—PCR assays, showed up to 10 potential mismatches with the Asian lineage causing the current outbreak, compared with 0 to 4 mismatches for the new assays. Conclusion We provide reagents and updated protocols for Zika virus detection suitable for the current outbreak strains. Introduction The Zika virus is a mosquito-borne flavivirus with an approximately 11 kilobase ribonucleic acid RNA genome.
Open in a separate window. Methods Assays We compared nine different assays. Table 1 Oligonucleotides used in Zika virus real-time reverse transcription polymerase chain reaction assays and potential nucleotide mismatches with Zika virus strains. Assay, reference Target genomic domain bases a No. Clinical specimens We obtained clinical specimens from travellers for which routine medical investigation of either Zika or dengue virus had been requested due to compatible clinical symptoms or a travel history to affected countries.
Results All assay-specific IVT and the uncRNA allowed comparable quantification of Zika virus RNA with a mean twofold deviation of results maximum deviation: sixfold , suggesting the ability to use these controls to generate comparable results even when different real-time RT—PCR assays are used in different laboratories.
Table 2 Analytical sensitivity of Zika virus real-time reverse transcription polymerase chain reaction assays. Threshold cycle variation when using different reaction conditions and thermocyclers. Viral loads of Zika virus in clinical specimens. Viral loads of Zika virus in paired urine and blood samples.
Risk of false-negative Zika virus test results. Risk of false-negative dengue virus test results. Discussion This study provides guidance on the choice of method for diagnosing Zika virus infections and a molecular control that enables the comparison of results between different laboratories and studies. Competing interests: None declared. References 1. Kuno G, Chang GJ. Full-length sequencing and genomic characterization of Bagaza, Kedougou, and Zika viruses. Arch Virol. N Engl J Med.
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